Every year Andre cooks an incredible soup for the royal family and the citizens of Dor. When soup is outlawed from the kingdom, Andre gets lost in a depression. However, he finally decides to make soup anyway, and this brings back the rain, the sun and the light in the kingdom.
When Andre needs inspiration, he calls upon the help of Boldo. Boldo is completely made of food and claims his way of cooking is far superior to Andre´s. When Despereaux needs help rescuing Princess Pea, Boldo leaps into action and brings Despereaux into the Dungeons.
Lester is Despereaux father. He doesn´t understand Despereaux thirst for adventure, and he is upset when he discovers Despereaux is doing poorly in school for not learning how to scurry or cower.
Antoinette is Despereaux mother. She is concerned when Despereaux fails to learn to scurry or to cower.
Despereaux was born smaller than all the other mice, but with large aeorodynamic ears. He has a thirst for adventure, much to the chagrin of the other mice. He is banished from Mouseworld and, after a series of events, Despereaux is able to emulate the knight in his favourite story and rescue the princess.
Furlough is Despereaux older brother. He is everything a mouse should be and his parents try to get Furlough to teach Despereaux how to cower and scurry to no avail.
Roscuro comes to the Kingdom of Dor and is excited when he hears of Soup day. Enchanted by the aroma of the delicious soup, Roscuro dangles above the Queen´s bowl when he accidentally falls inside. Having given the Queen a heart attack and causing soup to be outlawed in Dor, Roscuro spends his time in the kingdom trying to rid himself of the grief.
The leader of the rat world, Botticelli attempts to teach Roscuro how to be a proper rat. He holds coliseum-like battles in the arena and is shown to have little remorse for his actions. Roscuro blinds him by reflecting light from the sun and Despereaux traps him in the cage with the arena cat.
Miggery always was a Princess in her own mind. After being sold by her guardian, Miggery became Princess Pea´s servant girl. She longs to wear a crown and be a pretty pretty princess. Longing turned to anger, she agrees to help Roscuro kidnap the princess.
The princess longs for the rain, the soup and even the rats to return to the kingdom of Dor. She finds a friend in Despereaux, calling him ´´ Her Little Gentleman`` . She screams when Roscuro attempts to apologize on what happened on Soup Day which causes her to be kidnapped by an angry Roscuro and Miggery.
Smudge is a rat that lives in the sewers below the castle. He appears to be respected by the other rats, and he leads the pack by telling them when to strike.
Hovis is the threadmaster of Mouseworld. He ties thread to those banished from Mouseworld and lowers them into the sewers of Ratworld. He suports Despereaux´s way of life, telling him there is nothing to be ashamed of.
Gregory is the jailmaster beneath the castle in the kingdom of Dor. He rescues Miggery from her cell and notices the heart on the back of her neck. Gregory is overcome with happiness as he realizes Miggery is his daughter that he has not seen in ages.
After Despereaux failed to get the attention of the king when Princess Pea was in trouble, he had to go into action himself. Despereaux grabs a sewing needle and fends off attacking rats and saves Princess Pea from impending doom.
Chivalry, bravery, honor!
After being sent to Ratworld, Despereaux is pitted against this cat in the arena. The cat reappears later and is used by Despereaux to clear out the rats surrounding Princess Pea.
Every year, this soup pot is filled with delicious soup from Andre on a day called Soup Day. An extra ingredient is added to the soup accidentally when Boldo and Andre are having a food fight, but Andre is overjoyed when this soup pot in now filled with the perfect soup.